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KMID : 0377619710210030211
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Korean Jungang Medical Journal
1971 Volume.21 No. 3 p.211 ~ p.215
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Effect of Culture Media and Cell Treatments on the Heavy Metal Ion Reaction of Vibrio Cholera from Variants
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Abstract
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S-, SR-, and R-forms of Vibrio cholerae biotype El Tor strain Ogawa 41 were obtained after culturing the organism successively in immune serum-containing broth.
Influences of various culture media on the agglutination behaviour of the organisms were examined, using acrifiavine, lead acetate and manganous chloride as reacting agents.
Glucose (1%) had an effect of moderately diminishing the agglutination intensity and titer of SR-form with lead acetate, while lactose (1%) had the contrary effect, i. e., even SR-form becoming agglutinable in manganous chloride solution.
Trypsin treatment of the cells also brought about another interesting phenomenon; the differences between form variants in the agglutination degree with lead acetate were considerably dulled, even S-form becoming agglutinable while R-form loosing its spontanous agglutinability.
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